Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 500 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660121.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 500 Tablets per Bottle
PBS [Phosphate Buffered Saline] 200mL Tablets are designed for laboratories that need to prepare larger volumes of PBS quickly and accurately. Each tablet dissolves easily in water to create 200 mL of phosphate-buffered saline, a standard buffer used in many biological and biochemical applications. The 500-tablet bottle is perfect for high-throughput labs that require a consistent and reliable buffer solution. These tablets ensure that the PBS solution maintains the correct pH and osmolarity, which is essential for applications such as cell culture, protein assays, and molecular biology experiments. The tablets are convenient, cost-effective, and reduce the chance of errors in solution preparation.
FUJIFILM BIOSCIENCES INC MEM 1X Earles Salts w/o NEAA
MEM 1X Earles Salts w/o NEAA is an uncomplicated medium that is useful for a wide range of species and cells
CAPTIVATE BIO LLC DPBS no Cal. or Mag.
Captivate Bio s DPBS is a balanced salt solution used for various cell culture applications like cell washing transporting cells and diluting cells for counting This formulation is configured without calcium or magnesium Manufactured in the US under ISO and GMP conditions 12 x 1000mL
Miltenyi Biotec, Inc. CliniMACS PBS/EDTA Buffer research
Suitable for cell processing and cell separation in combination with the CliniMACS System.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
FUJIFILM BIOSCIENCES INC HBSS 10X-Hanks balan Salt sol
HBSS 10X Hanks Balanced Salt Solution is designed to maintain pH and osmotic balance and to provide cells with water and essential inorganic ions
Alkali Scientific CellPro™ DPBS Buffer, 1X, with Calcium and Magnesium, 1L, 10/Case
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Cayman Chemical oloyl EthnolamID phsphate 10mg
An LPA mimetic, LPA receptor agonist, and phosphate ester of OEA; selectively increases [35S]GTPγS binding to HEK293T cell membranes expressing LPA1 and LPA2 over LPA3 receptors; induces calcium mobilization and inhibits forskolin-induced cAMP accumulation in MDA-MB-231 cells (EC50s = 1.2 and 101 nM, respectively)
Boston Bioproducts Inc 10X Dulbecco's Phosphate Buffered Saline, DPBS (no Calcium, no Magnesium) - 2x1L
Bioprocess Grade - 10X Dulbecco s Phosphate Buffered Saline DPBS (no Calcium no Magnesium) - 2x1L (For Research Use and Further Manufacturing Only)
ILEX LIFE SCIENCES LLC TRYPSIN/EDTA P10-024100 100ML
PAN-Biotech (10x) Trypsin 0.5% / EDTA 0.2% in DPBS, w/o: Ca and Mg (100 ml). DESCRIPTION: PAN-Biotech (10x) Trypsin 0.5% / EDTA 0.2% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme plus EDTA in a 10x concentrated PBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.50% Trypsin. EDTA: 0.20% (5.3 mM) EDTA. STERILE: Yes. STORAGE TEMP.: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).